Antibody detection and sequencing analysis of Hepatitis E Virus in human population, swine and chicken in Sichuan region in China

VernacularTitle:四川地区部分人群和与人接触密切的家畜禽戊型肝炎病毒感染的调查及基因分型的研究
Author: Yong-Zhon JIANG ¹ ; Jian LU ; Li-Ping ZHANG ; Rui-Guang TIAN ; Qing-Lian LIU ; Sheng-Li BI
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Hepatitis E virus; Genotype; Epidemiology
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):468-471
CountryChina
Language:Chinese
Abstract: Objective To investigate the seroprevalence of HEV infection and genotype. Methods ELISA were used for detecting anti-HEV IgG of the serum samples,the nested reverse transcriptase PCR ( RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Siehuan province. The primers used for genotyping were the ORF2 region of HEV genome. Results The anti-HEV lgG was detected positive in childrens 6.10% (41/672),adults 42.26% (280/661 ),swines 88.89 % (32/36),chickens negative (0/59). 1 case of 15 serum samples of anti- HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR. Sequenee analysis of 4 isolates has 92.1% to 98.6% nucleofide sequence homology.These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology,which belonged to HEV genotype 4B. Conclusions The swine were the risk factors in the spread of hepatitis E virus.