Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction.
- Author:
Jian ZHOU
1
;
Guang JI
;
Jia-na WEN
;
Jia LI
;
Wei SHENG
;
Zhi-quan GUO
;
Guo-yang LIAO
;
Shu-de JIANG
;
Ming-bo SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biotechnology; methods; Cell Culture Techniques; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Hepatitis A Vaccines; metabolism; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity; Vaccines, Inactivated; metabolism; Viral Vaccines; metabolism; Virus Cultivation; methods; Virus Inactivation
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):488-491
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.
METHODSeffective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.
RESULTSall the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.
CONCLUSIONICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.
