Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-palymerase chain reaction

VernacularTitle:运用细胞培养/链特异性逆转录聚合酶链式反应法进行甲肝灭活疫苗的灭活验证试验
Author: Jian ZHOU ¹ ; Guang JI ; Jia-Na WEN ; Jia LI ; Wei SHENG ; Zhi-Quan GUO ; Guo-Yang LIAO ; Shu-De JIANG ; Ming-Bo SUN
Author Information

1. 中国医学科学院北京协和医学院医学生物学研究所
Keywords: Hepatitis A; Vaccius,inactivated; Cell culture; Reverse transcriptase-polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):488-491
CountryChina
Language:Chinese
Abstract: Objective To establish an quick,sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine. Methods effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR(ICC/strand-specifc RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay. Results all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR. Conclusion ICC/strand-specific RT-PCR assay is a novel,rapid,sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely,this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.