Research on an improved Lowry method to determine content of protein in Sabin IPV.
- Author:
Wei CAI
1
;
Ming-bo SUN
;
Hong-bo CHEN
;
Chao HONG
;
Xiao-yan SONG
;
Lei YU
;
Shu-de JIANG
;
Guo-yang LIAO
;
Jing-si YANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; metabolism; Calibration; Chemistry Techniques, Analytical; methods; Phenols; chemistry; Poliovirus Vaccine, Oral; chemistry; Proteins; analysis; Trichloroacetic Acid; chemistry
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):495-497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the content determination of protein in Sabin IPV.
METHODSUsing lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.
RESULTSThe method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.
CONCLUSIONThe method can be used to determine the micro content of protein in vaccines.
