Detection of influenza viruses/avian influenza viruses and identification of virulence using a microarray.
- Author:
Fei JIA
1
;
Rong-bao GAO
;
Min WANG
;
Yuan-ji GUO
;
Le-ying WEN
;
Ye ZHANG
;
Yan-hui CHENG
;
Yue-long SHU
;
Hong-sheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Birds; Humans; Influenza A virus; genetics; isolation & purification; pathogenicity; Influenza in Birds; virology; Influenza, Human; virology; Microbiological Techniques; Oligonucleotide Array Sequence Analysis; methods; Orthomyxoviridae; genetics; isolation & purification; pathogenicity; RNA, Viral; analysis; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Virulence; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):501-503
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the DNA microarray to detect influenza viruses and avian influenza viruses, and identify their virulence.
METHODSHemagglutinin (HA), neuramidinase (NA) and nucleoprotein(NP) genes were chosen simultaneously as targets for designing a microarray used for detection of viruses and identification virulence. The nucleic acid were amplified by single primer amplication (SPA). And then its specificity,sensitivity and reproducibility were evaluated.
RESULTSThe microarray was able to specially detect H1N1, H3N2, B influenza viruses and H5N1, H9N2 avian influenza viruses. Their limits were 8HAU, 16HAU, 32HAU, and 8HAU, 8HAU respectively. The limit for virulence was 32HAU. When samples were analyzed by both RT-PCR and microarray in parallel, the results agreed in 83.9% (47/56).
CONCLUSIONThe microarray can detect and distinguish five tested viruses, and especially identify virulence. It not only supplies an assistant tool for clinical diagnosis and control of infectious disease, but also is valuable for controlling and preventing outbreak of avian influenza epidemic.
