Detection of influenza viruses/avian influenza viruses and identification of virulence using a microarray
10.3760/cma.j.issn.1003-9279.2008.06.034
- VernacularTitle:流感/禽流感病毒及其致病力鉴别的基因芯片技术研究
- Author:
Fei JIA
1
;
Rong-Bao GAO
;
Min WANG
;
Yuan-Ji GUO
;
Le-Ying WEN
;
Ye ZHANG
;
Yan-Hui CHENG
;
Yue-Long SHU
;
Hong-Sheng LIU
Author Information
1. 辽宁大学
- Keywords:
Influenza virus;
Avian influenza virus;
DNA microarray;
Virulence
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(6):501-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the DNA microarray to detect influenza viruses and avian influenza viruses,and identify their vindence. Methods Hemagglutinin(HA) ,neuramidinase(NA) and nuclooprotein(NP) genes were chosen simultaneously as targets for designing a microarray used for detection of viruses and identification virulence. The nucleic acid were amplified by single primer amplication (SPA). And then its specificity,seusitivity and reproducibility were evaluated. Results The microarray was able to specially detect H1N1 ,H3N2,B influenza viruses and H5N1,H9N2 avian influenza viruses. Their limits were 8HAU,16HAU,32HAU,and 8HAU,8HAU respectively .The limit for virulence was 32HAU. When samples were analyzed by both RT-PCR and microarray in parallel,the results agreed in 83.9% (47/56).Conclusion The microarray can detect and distinguish five tested viruses,and especially identify virulence. It not only supplies an assistant tool for clinical diagnosis and control of infectious disease,but also is valuable for controlling and preventing outbreak of avian influerza epidemic.
