Atomic force microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40.
- Author:
Kong-xin HU
1
;
Li-ping ZHANG
;
Chao MI
;
Yan-fei LIU
;
Hua-qian SHUO
;
Jing WANG
;
Le ZHANG
;
Bao-lin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Influenza A virus; chemistry; drug effects; Microscopy, Atomic Force; instrumentation; Nanostructures; Orthomyxoviridae; chemistry; drug effects; Polyethylene Glycols; pharmacology; Spermatocidal Agents; pharmacology
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(6):507-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThrough observing the morphology and topography of the prepared influenza viruses (H1N1) treated with the different Nonidet P-40 solutions using atomic force microscopy (AFM), to explore the application of AFM on the research of the internal character of viral morphology and structural virology.
METHODSThe virus samples were treated with serial diluted Nonidet P-40 solutions from 0.05% to 0.20% and then investigated by AFM with the tapping mode in air at room temperature to obtain the morphology and topography changes including height data,amplitude data and phase data for both spherical and filamentous influenza virus A.
RESULTSThe serial AFM images show that the erosion degree of the virions is proportional with the improvement of NP-40 concentration,and partly denuded virion image appeared at 0.05% NP-40 treatment, which was revealed clearly on both amplitude images and phase images.
CONCLUSIONThis work demonstrated for the first time that the internal topography of influenza virion could be revealed by AFM via suitable nonionic surfactants chemical dissection.
