Investigation on distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai.
- Author:
Jie ZANG
1
;
Shuang-Ying JIANG
;
Xue-Wen WANG
;
Feng WANG
;
Tao BIAN
;
Zhen-Yun MU
;
Yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; China; Genotype; Hepatitis Antibodies; blood; Hepatitis B; immunology; virology; Hepatitis B Core Antigens; genetics; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; classification; genetics; immunology; isolation & purification; Humans; Male; Middle Aged; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):2-4
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai.
METHODSNested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result.
RESULTS271, which come from 311 sera samples with positive HBsAg randomly selected from natural community, were amplified and sequenced in both S gene and C gene successfully, 10 (3.7%), 261 (96.3%) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7%), 2 (0.7%) belonged to serotype ayw2, adr, adw2 respectively.
CONCLUSIONThe recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis Bin Tongde, Qinghai; the serotype of this areas was consisted largely of ayw2.
