Investigation on distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghat

VernacularTitle:青海同德藏族人群感染HBV基因型、血清型调查
Author: Jie ZHANG ¹ ; Shuang-Ying JIANG ; Xue-Wen WANG ; Feng WANG ; Tao BIAN ; Zhen-Yun MU ; Yong ZHANG
Author Information

1. 河北医科大学
Keywords: Hepatitis B virus; Genotype; Serotyping
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):2-4
CountryChina
Language:Chinese
Abstract: Objective To explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai. Methods Nested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result. Results 271, which come from 311 sera samples with positive H BsAg randomly selected from natural community, were amplified and sequenced in beth S gene and C gene successfully, 10(3.7 % ), 261 (96.3 % ) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7 % ), 2 (0.7%) belonged to serotype ayw2,adr,adw2 respectively. Conclusion The recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis B in Tongde, Qinghai;the serotype of this areas was consisted largely of ayw2.