The analysis of "alpha" dominant mutation of hepatitis B virus in community-based Zhengding area, Hebei province.
- Author:
Hong-Xia YAN
1
;
Jing-Chen MA
;
Tao BIAN
;
Chang-Hong HUANG
;
Shuang ZHANG
;
Zhi-Yong ZHANG
;
Zhi-Yong HAO
;
Ji-Chao CHEN
;
Yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Female; Hepatitis B; virology; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; genetics; isolation & purification; Humans; Male; Middle Aged; Mutation; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the "alpha"dominant mutation of hepatitis B virus (HBV) in community-based Zhengding. Analysis the role of the newborn hepatitis B vaccination on the mutation.
METHODSBased on the national surveillance of hepatitis B, 11,478 people's sera were collected and tested by SPRIA with kits. Collect people's sera with positive HBsAg and amplify the S gene. Sequencing and clastwaling them with the standard sequences.
RESULTSOverall, HBV DNA was successfully amplified and sequenced in 434 of 443 samples. 6.7% samples mutated in HBV "alpha" dominant region. The difference between the mutation ratio of the two loops of HBV "alpha" dominant between the people born before and after the year 1986 has no significance.
CONCLUSIONThere were HBV "alpha" dominant mutant virus in the local area with a low infection rate in the population born after the year 1986. It could not explain the newborn hepatitis B vaccination can induce the prevalence of the "alpha" dominant mutate HBV.
