Study of Gag gene antigen epitypes variation and the quasispecies group characteristics in Henan area HIV-1 strains

VernacularTitle:河南地区HIV-1毒株Gag基因抗原表位变异特征及准种特点研究
Author: Peng DU ¹ ; Guo-Min CHEN ; Ze-Lin LI ; Yi ZENG
Author Information

1. 中国疾病预防控制中心病毒病所
Keywords: HIV-1; Antigen epitypes; Quasispecies group
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):20-22
CountryChina
Language:Chinese
Abstract: Objective To study the characteristics of the variation of antigen epitopes and quasispecies group in the HIV-1 viruses in Henan province in China. Methods The region of the p17-p24 of the HIV-1 gag gene was amplified by nested polymerse chain resction(PCR), purified products were cloned into the vector, the obtained were analyzed by MEGA soft wares. Results B' subtype strains were predominant in Henan province, the mutations in antigen epitypes of the p17 region of the gag gene focus on E62g(55.8% ) ,Y79f(48.9% ) ,T84V (48.9), I44V(44.2%), the p24 region had not found the distinct mutation. Conclusion Both the PCR sequences and clone strains sequences demonstrated that four antigen epitopes mutations in p17 region of the HIV B' subtype gag gene, and the region of p24 was more conservative,which was suitable for development of the epitope vaccien.