The infection of dendritic cells by recombinant adenoviral vector carrying HBsAg-HSPT0 chimeric gene and Its biological characteristics observations

VernacularTitle:腺病毒介导HSP70-HBsAg嵌合基因转染树突状细胞及生物学特性分析
Author: Chun-Liang LEI ¹ ; Yang-Ling OU ; Zhan YANG ; Xiao-Ping TANG
Author Information

1. 广州市第八人民医院
Keywords: Heat-Shock proteins 70; Hepatitis B virus; Dendritic cells; Adenoviruses,human
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):29-31
CountryChina
Language:Chinese
Abstract: Objective To test the infeciton efficiency of recombinant adenovixal vector carrying HBsAg-HSP70 chimeric gene and to abserve its biological characteristics. Methods Peripheral blood mononuclear cells (PBMC) were separated from healthy blood donor and they were infected by Ad-HSP70-HBsAg on the first day of isolation. DCs were induced in medium with cytokines IL-4, GM-CSF and TNF-α in vitro. The biological characteristics of DC induced were analyzed by inverted fluorecent microscope, RT-PCR, flow cytometer(FACS), and mixed lymphocyte reaction (MLR). Results The traced gene-GFP were abserved in DCs by inverted fluorecent microscope and HSP70-HBsAg gene mRNA expression was detected by RT-PCR after the Ad-HSP70-HBsAg infection. FACS analysis shown that the expression of CD1a,CDSO,CD86 and HLA-DR on surfece of two groups of DCs were similar. MLR showed that there are not a statitie difference of stimulated index (SI) between two groups.Conclusion Results indicated that Ad-HSP70-HBsAg can effectively infected DCs without affecting its biological characteristics.