In vitro study on the effect of Ribavirin reducing the EV71 replication
10.3760/cma.j.issn.1003-9279.2009.01.016
- VernacularTitle:利巴韦林注射液在细胞水平上抑制EV71病毒复制的初步研究
- Author:
Yan ZHANG
1
;
Zhen ZHU
;
Shuang-Li ZHU
;
Hong-Qiu AN
;
Zhoo-Hui YANG
;
Xue-Bin GUO
;
Wen-Bo XU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Enterovirus;
Ribavirin;
in vitro
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(1):44-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro. Methods Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed. Results In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group. Conclusion Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.
