Application of 293 cells in in vitro detection of carcinogens

VernacularTitle:293细胞检测致癌物的应用研究
Author: Rui ZHAO ¹ ; Lei ZHANG ; Ru-Gang ZHONG ; Ling ZHOU ; Yi ZENG
Author Information

1. 北京工业大学
Keywords: Cell lines; Microcystin LR; Cell transformation,neoplastic; Carcinogens
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):47-49
CountryChina
Language:Chinese
Abstract: Objective To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study. Methods The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Micrecystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to defme the promotive activity of 293 cells. Results 293 cell acted like tumor cells after induced by MC-LR: serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice. Conclusion 293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicions carcinogens.