Peripheral NK and NKT cell in pediatric subjects with chronic HBV infection

VernacularTitle:HBV感染患儿外周血NK及NKT细胞分析与临床意义
Author: Bin YANG ¹ ; Li-Ming CHEN ; Wen-Hua YE ; Hong-Fei ZHANG ; Yun CHENG
Author Information

1. 解放军第302医院
Keywords: Child health Services; Hepatitis B Virus; Killer Cells,natural; Flow cytometry
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):59-61
CountryChina
Language:Chinese
Abstract: Objective To investigate the characteristic of NK cells and NKT cells in HBV infected pediatric subjects for evaluation of their clinical implication. Methods Fresh peripheral blood samples were obtained from 42 HBV-infeeted pediatric cases and 15 healthy counterparts. NK cells and NKT cells were analyzed by flow cytometry assay. The ehnical data such as serum ALT level and HBV viral load was simultaneously recorded from each HBV cartier. Results HBV-infected children had an obviously increasing percentage of NK cells 12.071% ± 7.100%, there were significant differences between the children with chronic B hepatitis and the healthy children ( P < 0.05). As far as percentage of NKT 3.048% ± 1.937% was concerned, there were not differences. Furthermore the association was not found between serum HBV viral load level and the NK lymphocyte. Conclusion Our data may provide valuable information of NK .and NKT lymphocyte for evaluation of disease progression of HBV infected children NKI cells.