Preparation and identification of the monoclonal antibodies against VP1 capsid protein of Enterovius 71

VernacularTitle:抗肠道病毒EV71型外壳蛋白VP1单克隆抗体的制备和鉴定
Author: Yao YI ¹ ; Min-Zhuo GUO ; Xin-Liang SHEN ; Tao YU ; Zhi-Yuan JIA ; Sheng-Li BI ; Xiu-Ling LI ; Si-Yong CHEN
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Entorovirus; Antibodies,monoclonal; Enzyme-hnked immunosorbent assay
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):62-64
CountryChina
Language:Chinese
Abstract: Objective To prepare monoclonal antibodies (McAbs) against VP1 capsid protein of Enterovims 71. Methods Two peptides, SP55 and SPT0, containing amino acid 163-177 and 208-222 of VP1, were synthesized respectively. Immunized BALB/c mice with the synthetic peptides to estabhsh the hybridoma cell strains secreting specific McAb to VP1. After the specific McAbs were prepared, identified and analyzed the titer by indirect ELISA assay. The positive clones were selected and their neutralization titer were determined by neutralization test. Results Two high titered anti-VP1 antibodies secreted by the hybfidoma cells showed good neutralization reaction with enterovirus 71 on RD cells, and the neutralization titer were 1:8 and 1 : 16 respectively. Conclusion Two high titered anti-VP1 antibodies, with good neutralization activity, secreted by the hybridoma cells, which lays the foundation for further study.