Improve on serological diagnosis method of nasopharyngeai carcinoma
10.3760/cma.j.issn.1003-9279.2009.01.023
- VernacularTitle:鼻咽癌血清学检测方法的改进
- Author:
Shao-Bing ZHAN
1
;
Jian-Ming ZHONG
;
Zhi-Ping MAI
;
Shu-Qing YE
;
Ling ZHOU
;
Yi ZENG
;
Jian LIAO
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Herpesvirus 4,humen;
Nasopharyngeal neoplasms;
Streptavidin;
Biotin
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(1):65-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the existing serological early diagnosis method of nasopharyngeal carcinoma by improve the detection sensitivity. Methods The samples of 294 serum specimen from the prevention and treatment of nasopharyngeal cancer model base, involving 106 serum specimen from the patients suffering from nasopharyngeal cancer and 188 from the healthy testers. IgA/VCA antibody and IgA/EA antibody of the serums are tested by Streptavidin-biotin-antibody immunoenzymatic test and normal traditional enzyme methods, SPSS statistical software is used to analyse the test results with χ2 test and t test. Results Referring to 106 patients, the sera antibody positive rate tested by Streptavidin-biotin-antibody immunoenzymatie test method is obviously higher than that tested by traditional method;and the t test result of the GMT has significant difference in the two method. Conclusion The modified method can improve the sensitivity of serology testing, ensure the specificity of test results, at the same time, improve the detection rate of nasopharyngeal carcinoma, so it can be applied to the early screen work of nasopharyngeal carcinoma.
