Preparation and Characterization of monoclonal antibodies to rabies virus CVS-11 strain

VernacularTitle:抗狂犬病病毒CVS-11株单克隆抗体的制备及鉴定
Author: Lei CAO ¹ ; Xiong LI ; Shou-Feng ZHANG ; Liu-Jing LI ; Rong-Liang HU ; Qing TANG
Author Information

1. 第四军医大学
Keywords: Rabies virus; Antibody,mononclonal
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):68-70
CountryChina
Language:Chinese
Abstract: Objective To get the high specific and sensitive mononclonal antibodies against RV. Methods The myeloma cell line SP2/0 fused with the spleen cell of 6 - 8 weeks old BALB/c mice immunized with the CVS-11 virus antigen. The hybridized fusing cells were chosen by indirect ELISA detection and the positive hybridizing cells were amplified through mouse abdomen injection and the mouse McAbs ascites was purified by Protein A Sepharose 4 Fast Flow(Pharmacia Company). The specificity and sensitivity of the McAbs was identified by indirect ELISA and indirect DFA test. Results The cell fusion rate reachs 100% and the indirect ELISA results showed that the McAbs ascites titer were 1 ×104,1 ×105, 1×104 and 1×105;The immunoglobulin G type McAbs show no cross reaction with other related viruses. Conclusion The high specific and sensitive mononclonal antibodies of RV can be used for rapid RV diagnosis.