Evaluation of the ELISA and the enhanced chemiluminescence immunoassay in use to determine the serum markers for liver fibrosis.
- Author:
Jing-Xia GUO
1
;
Hong-Bin MA
;
Yong-Li LI
;
Jun XU
;
Li-Hua YANG
;
Jia-Bin SHI
;
Bo LI
;
Ai-Xia LIU
;
Xiao-Xi LI
;
Bo-An LI
;
Yuan-Li MAO
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers; blood; Collagen Type III; blood; Collagen Type IV; blood; Enzyme-Linked Immunosorbent Assay; methods; Humans; Laminin; blood; Liver Cirrhosis; blood; diagnosis; pathology; Luminescent Measurements; methods
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):71-73
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the detection method of ELISA and Enhanced Chemiluminescence Immunoassay (ECLIA) in use to determine serum hyaluronate acid (HA), laminin (LN), type IV collagen (IV-C) and type III procollagen (PC III).
METHODS253 patients with chronic hepatitis B were determined the four liver fibrosis serum markers with both the ECLIA and ELISA, and then compared with pathology results separately.
RESULTSBoth the detection results of ELISA and ECLIA can reflect that the patient's liver fibrosis from hepatitis to liver cirrhosis aggravated gradually. Compared with ELISA, the results of ECLIA and pathology have a better correlation.
CONCLUSIONSThe detection of four liver fibrosis serum markers by ECLIA could indicate the better the response of the state of live fibrosis.
