Evaluation of the ELISA and the Enhanced Chemiluminescence Immunoassay in use to determine the serum markers for liver fibrosis

VernacularTitle:血清肝纤维化指标酶联免疫分析实验和增强化学发光免疫分析系统检测的评价
Author: Jing-Xia GUO ¹ ; Hong-Bin MA ; Yong-Li LI ; Jun XU ; Li-Hua YANG ; Jia-Bin SHI ; Bo LI ; Ai-Xia LIU ; Xiao-Xi LI ; Bo-An LI ; Yuan-Li MAO
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1. 解放军第302医院
Keywords: Liver cirrhosis; Serology; Chemiluminescence; Enzyme-linked immnoserbent assay
From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):71-73
CountryChina
Language:Chinese
Abstract: Objective To evaluate the detection method of ELISA and Enhanced Chemiluminescence Immunoassay (ECLIA) in use to determine serum hyaluronate acid (HA), hminin (LN), typeⅣ collagen (IV-C) and type Ⅲ procollagen (PC Ⅲ ). Methods 253 patients with chronic hepatitis B were determined the four liver fibrosis serum markers with both the ECLIA and ELISA, and then compared with pathology results separately. Results Both the detection results of ELISA and ECLIA can reflect that the patient's liver fibrosis from hepatitis to liver cirrhosis aggravated gradually. Compared with ELISA, the results of ECLIA and pathology have a better correlation. Conclusions The detection of four liver fibrosis serum markers by ECLIA could indicate the better the response of the state of live fibrosis.