The comparison of the three anti-dsDNA antibody detecting test.
- Author:
Da-Wei TONG
1
;
Shu-Lan ZHANG
;
Yong-Zhe LI
;
Chao-Jun HU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Antinuclear; analysis; immunology; Case-Control Studies; DNA; immunology; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique, Indirect; Humans; Immunologic Tests; methods; Lupus Erythematosus, Systemic; diagnosis; immunology; Radioimmunoassay
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(1):74-75
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the three Anti-dsDNA antibody detecting test (IIF, ELISA, Farr) with 200 serum samples to evaluate which one has higher sensitivity and specificity.
METHODS200 serum samples including 120 serum samples of SLE, 20 serum samples of rheumatoid arthritis, 20 serum samples of MCTD, 20 serum samples of SS, 20 serum samples of PSS and 50 serum samples of healthy measured by IIF, Farr and ELISA.
RESULTSDetection the Anti-dsDNA antibody of the serum sample with the methods of IIF, ELISA and Farr. The positive percentage of Anti-dsDNA in SLE is 25%, 32% and 32%, while in RA is 0, 0 and 0; in PSS is 0, 0 and 5%; in SS is 0, 0 and 0; in healthy is 0, 0 and 0.
CONCLUSIONDetection the Anti-dsDNA antibody with two method in the same time, especially with IIF and ELISA, will heighten the positive rate than with single method and will be helpful for the diagnosis of SLE.