Gene characterization of E6 and E7 gene of human papillomavirus of 15 cervical cancer in Beijing
10.3760/cma.j.issn.1003-9279.2009.02.004
- VernacularTitle:人乳头瘤病毒16型E6和E7基因特征分析
- Author:
Zhuo REN
1
;
Hong-Wei WU
;
Jie SONG
Author Information
1. 北京市海淀区妇幼保健院
- Keywords:
Esophageal neoplasmas;
Adenocarcinoma;
Careinoma,squamous cell;
Papillomavirus,human;
Genes
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(2):88-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective Conducting the gene characterization of the E6 and E7 gene of human papillomaviros 16(HPV16)isolated from 15 cases of cervical cancer at Beijing. Methods Overlapping primers were designed according to the full-length genomes of E6 and E7 from the GenBank and PCR was used to amplify the E6 and E7 fragments. TA clone was used to select a purified clone in order to have better and valuable sequencing results. Nucleotide and amino acid sequence were analyzed by the Sequencer, Bioedit, Mega et al. Results 8 of 15 (8/15) cervical samples contained HPV E6 and E7 gene, and 4 had Asian type like and 4 had Europe prototype like. There were two nucleotide mutation at E6 position 178 (T→G,D25E) and at E7 position 647 (A→G, N29S) in 4 Asian type like viruses. There were one nucleotide mutation at E6 position 335 (C→T, H78Y) in 1 of 4 Europe prototype like virus. In the cervical cancer samples, 8 of 15 contained the HPV16E 6 and E7 gene. HPV16 E6 and E7 can not be detected in denosquamous carcinoma and adenocarcinoma. Conclusion HPV16 is the main etiology of the cervical carcinoma. The HPV16 infectious ratio of squamous carcinoma is more than the ratio of adenocarcinoma. 178th nucleotide in E6 gene is the very important site to distinguish the Asia and the Europe prototype strain like. 178 nueleotide in E6 and 647 nucleotide in E7 are the frequent mutation site in cervical carcinoma. Analysis based on the E6 and E7 gene sequence of HPV 16 isolates suggests that naturally occurring sequence variants of E6 and E7 gene may have identify the oncegenie properties.
