Construction of human metapneumovirus DNA vaccine and study on its immune response in mice LIU
10.3760/cma.j.issn.1003-9279.2009.02.008
- VernacularTitle:人偏肺病毒DNA疫苗的构建和小鼠免疫反应的研究
- Author:
Pei WEN
1
;
Li-Shu ZHENG
;
Zhao-Jun DUAN
;
Zhi-Ping XIE
;
Qian ZHANG
;
Wan-Ju ZHANG
;
Yun-De HOU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Metapneumovirus;
Vaccine,DNA;
Immunity,cellular;
Antibody formation
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(2):100-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct human metapneumovirns (hMPV) DNA vaccines and evaluate the cellular and humoral immune response in mice.Methods Fusion protein FATM (without transmembrane domain) gene and M gene of hMPV were amplified from cDNA by PCR, then DNA vaccines pcDNA3. 1His-FΔTM and peDNA3.1His-M were constructed to verify the expression of F and M protein by Western blotting and indirect immunofluorescent assay (IFA) respectively. Serum IgG and spleen cell CTL were detected with ELISA and ELISPOT assay after the BALB/c mice were immunized intramuscularly with the vaccines. Results The candidate DNA vaccines could express FATM and M protein as detected with Western blotting and IFA. The IgG antibody titers of mice was 1:44 when immunized with poDNA3.1His-FΔTM, but could increase te 1:64 when co-immunized with pcDNA3.1His-M. ELISPOT assay demonstrated that IFN-γ-secreting effector T cells reached 42 ±8.9 in co-immunization group, higher than single vaccine pcDNA3.1His-FΔTM group (32 ± 7.4). Conclusion DNA vaccine pcDNA3.1His-FΔTM could induce specific cellular and humoral immune responses, and the immune response could increase when co-immunization with pcDNA3.1His-M was carried out.
