Genetic characterization of the N protein of subgroups A and B human respiratory syncytial viruses.
- Author:
Yan ZHANG
1
;
Hui-ling WANG
;
Zheng-de XIE
;
Xiao-hui KONG
;
Chun-yan LIU
;
Kun-ling SHEN
;
Wen-bo XU
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Molecular Sequence Data; Nucleocapsid Proteins; chemistry; genetics; Phylogeny; Respiratory Syncytial Virus Infections; virology; Respiratory Syncytial Virus, Human; chemistry; classification; genetics; isolation & purification; Sequence Homology, Amino Acid
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(2):115-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.
METHODSReverse transciption polymerase chain reaction (RT-PCR) was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004. The RT-PCR products were sequenced for N protein coding region. The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.
RESULTSThe differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36-40 (3.1%-3.4%) and 4 (1.0%). The differences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17 (1.4%) and 1 (0.3%). The differences in number of nucleotide and deduced amino acid were 3-172 (0.25%-14.63%) and 0-18 (0-4.6%) respectively between 4 Beijing 2004 isolates and GenBank sequences.
CONCLUSIONN gene is the highly conservative gene in the HRSV genome. The variation between A and B subgroups were widely distributed in the entire gene of N protein, while the variation within the A or B subgroups HRSV was considerably lower.
