Genetic characterization of the N protein of subgroups A and B human respiratory syncytial viruses
10.3760/cma.j.issn.1003-9279.2009.02.113
- VernacularTitle:呼吸道合胞病毒分离株N蛋白编码基因特征分析
- Author:
Yan ZHANG
1
;
Hui-Ling WANG
;
Zheng-De XIE
;
Xiao-Hui KONG
;
Chun-Yan LIU
;
Kun-Ling SHEN
;
Wen-Bo XU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Respiratory Syncytial Viruses;
Genes;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(2):115-117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.Methods Reverse transciption polymerage chain reaction(RT- PCR)was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004.The RT-PCR products were sequenced for N protein coding region.The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.Results The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36.40(3.1%-3.4%)and 4(1.0%).The difierences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17(1.4%)and 1(0.3%).The differences in number of nucleotide and deduced amino acid were 3-172(0.25%-14.63%)and 0-18(0-4.6%)respectively between 4 Beijing 2004 isolates and GenBank sequences.Conclusion N gene is the hishly conservative gene in the HRSV genome.The variation between A and B subgroups were widely distributed in the entire gene of N protein,while the variation within the A or B subgroups HRSV wag considerably lower.
