An improved BALB/c 3T3 cell transformation assay and its application in the cocarcinogenesis study.
- Author:
Lei ZHANG
1
;
Rui ZHAO
;
Ru-gang ZHONG
;
Ling ZHOU
;
De-chang LI
;
Yong-ning WU
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: 3T3 Cells; Animals; BALB 3T3 Cells; Biological Assay; methods; Carcinogens; toxicity; Cell Transformation, Neoplastic; chemically induced; Cocarcinogenesis; Male; Mice; Mice, SCID; Random Allocation
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(2):121-123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve the protocol of BALB/c 3T3 cell transformation assay, and apply it to the cocarcinogenesis study.
METHODAppropriate serum concentration, culture media and method of administration were selected by testing their effects on the growth and transformation of BALB/c 3T3 cells. The co-carcinogenic activity between diethylnitrosamine (DEN) and microcystin-LR (MC-LR) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were examined using the improved cell transformation assay. The malignant characteristics of transformed cells were verified by neoplasia in SCID mice.
RESULTSThere were strong co-carcinogenic activity between DEN and TCDD. On the contrary, although MC-LR has strong ability to induce cell transformation, the effect was markely inhibited by DEN. The transformed cells show some malignant characteristics.
CONCLUSIONThe improved BALB/c 3T3 cell transformation assay is reliable and time-saving, and can be efficiently used in the study of cocarcinogenesis.
