An improved BALB/c 3T3 cell transformation assay and Its application in the cocarcinogenesis study

VernacularTitle:BALB/c 3T3细胞转化实验的优化及其在致癌物协同作用研究中的应用
Author: Lei ZHANG ¹ ; Rui ZHAO ; Ru-Gang ZHONG ; Ling ZHOU ; De-Chang LI ; Yong-Ning WU ; Yi ZENG
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Cell line; Cell transformation,viral; Cocarcinogenesis; Mice,inbred CALB/c
From: Chinese Journal of Experimental and Clinical Virology 2009;23(2):121-123
CountryChina
Language:Chinese
Abstract: Objective To improve the protocol of BALB/c 3T3 cell transformation assay, and apply it to the cocarcinogenesis study. Method Appropriate serum concentration, culture media and method of administration were selected by testing their effects on the growth and transformation of BALB/c 3T3 cells. The co-carcinogenic activity between diethylnitrosamine (DEN) and microcystin-LR (MC-LR) or 2,3,7,8-tetrachloredibenzo-p-dioxin (TCDD) were examined using the improved cell transformation assay. The malignant characteristics of transformed cells were verified by neoplasia in SCID mice. Results There were strong co-carcinogenic activity between DEN and TCDD. On the contrary, although MC-LR has strong ability to induce cell transformation, the effect was markely inhibited by DEN. The transformed cells show some malignant characteristics. Conclusion The improved BALB/c 3T3 cell transformation assay is reliable and time-saving, and can be efficiently used in the study of cocarcinogenesis.