Fluorescent microbeads-based multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.
- Author:
Jian-dong LI
1
;
Shuo ZHANG
;
Quan-fu ZHANG
;
Qin-zhi LIU
;
Yan WEI
;
Chuan LI
;
Mi-fang LIANG
;
De-xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Fluorescence; Hemorrhagic Fevers, Viral; blood; immunology; virology; Humans; Immunoassay; methods; Immunoglobulin M; blood; Microspheres; Nucleocapsid Proteins; chemistry; immunology; Viruses; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(2):149-151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.
METHODSThe nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits.
RESULTSFor detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously.
CONCLUSIONA robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.
