Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene.
- Author:
Xin-Chun CHEN
1
;
Wei-Long LIU
;
Gui-Lin YANG
;
Yong-Jun LIU
;
Xiu-Yun ZHU
;
Hong-Mei ZHANG
;
Bo-Ping ZHOU
;
Lonnie LYBARGER
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Animals; Cell Line; Epitopes, T-Lymphocyte; genetics; metabolism; Gene Expression; Genes, Reporter; Genetic Vectors; genetics; metabolism; Green Fluorescent Proteins; genetics; metabolism; H-2 Antigens; genetics; metabolism; Hepatitis B Surface Antigens; genetics; metabolism; Histocompatibility Antigen H-2D; Humans; Mice; Recombinant Fusion Proteins; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):161-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope.
METHODSAn oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT.
RESULTSHBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay.
CONCLUSIONA recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.
