Detection of minimal residual disease in childhood acute lymphoblastic leukemia by using real-time quantitative PCR.
- Author:
Ya-Ting ZHANG
1
;
Zhao-Fan LUO
;
Jian-Pei FANG
;
Hai-Xia GUO
;
Ke HUANG
;
Chi-Kong LI
Author Information
1. Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
DNA Primers;
Female;
Gene Rearrangement;
Genes, T-Cell Receptor;
Humans;
Male;
Neoplasm, Residual;
diagnosis;
genetics;
Polymerase Chain Reaction;
methods;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
diagnosis;
genetics
- From:
Journal of Experimental Hematology
2010;18(5):1235-1239
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to detect the minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) by using real time quantitative PCR (RQ-PCR) . The Ig and TCR gene rearrangements were amplified by using 18 primer sets in B-ALL, 8 primer sets in T-ALL; the ALL-MRD levels were quantified by using RQ-PCR with SYBR green dye staining and clone specific Ig/TCR gene rearrangements as molecular markers. The results indicated that there were 8 cases showing gene rearrangements in 9 B-ALL patients, marker detection rate for all samples was 88.8%, the MRD level on day 33 during induction treatment decreased significantly. It is concluded that Ig/TCR gene rearrangements can be used as a marker to detect MRD in childhood ALL; the technique of QR-PCR with SYBR green dye staining is reliable, relatively sensitive and easy performable method which can be used in routine detection for childhood ALL.
