Lysophosphatidic acid acyltransferase β gene expression in newly diagnosed leukemia patients.
- Author:
Rong ZHAN
1
;
Hao-Bo HUANG
;
Shun-Quan WU
;
Jun LIN
Author Information
1. Department of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China. deanzhanrong@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Acyltransferases;
genetics;
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Case-Control Studies;
Child;
Female;
Gene Expression;
Humans;
Leukemia;
genetics;
pathology;
Male;
Middle Aged;
RNA, Messenger;
genetics;
Young Adult
- From:
Journal of Experimental Hematology
2010;18(6):1422-1426
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to quantitatively detect the expression level of lysophosphatide acid acyltransferase β (lpaat β) mRNA in leukemia patients so as to provide theoretical basis for the target therapy of lpaat β in leukemia. Real-time fluorescently quantitative PCR was used to detect the relative expression level of lpaat β mRNA to analyze its expression change in various type of leukemia. The results showed that the lpaat β mRNA expression level in acute leukemia (AL) patients was significantly higher than that in normal controls (p < 0.05); lpaat β mRNA expression level in acute myeloid leukemia (AML) patients was significantly higher than that in normal controls (p < 0.05) and was positively correlated with white blood cell count (≥ 20.0 × 10(9)/L) (p < 0.05) and CD34 expression level of leukemia, but was not related with extramedullary infiltration. Except for acute promyelocytic leukemia (APL), the lpaat β mRNA expression level was negatively correlated with chemotherapy sensitivity in chronic myeloid leukemia (AML) patients. lpaat β mRNA expression level in chronic myeloid leukemia (CML) patients was significantly higher than that in normal controls (p < 0.05). lpaat β mRNA expression level in acute lymphoid leukemia (ALL) patients was not higher than that in normal controls (p > 0.05). It is concluded that the lpaat β gene overexpression exists in both AML and CML patients. lpaat β produced by AML cells probably plays an important role in abnormal proliferation and drug-resistance of AML cells.
