Effect of sodium valproate on human myelodysplastic syndrome cell line SKM-1 and its mechanism.
- Author:
Bao-An CHEN
1
;
Bo ZHANG
;
Chun-Rui LI
;
Jian-Feng ZHOU
;
Xue-Qiong WU
;
Chong GAO
;
Feng GAO
;
Guo-Hua XIA
;
Ze-Ye SHAO
;
Jia-Hua DING
;
Gang ZHAO
;
Jian CHEN
;
Jun WANG
;
Hui-Hui SONG
;
Wen BAO
Author Information
1. Department of Hematology, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China. cba8888@hotmail.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
CASP8 and FADD-Like Apoptosis Regulating Protein;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Intercellular Signaling Peptides and Proteins;
metabolism;
Membrane Proteins;
metabolism;
Myelodysplastic Syndromes;
metabolism;
pathology;
RNA, Messenger;
metabolism;
Valproic Acid;
pharmacology
- From:
Journal of Experimental Hematology
2010;18(6):1515-1519
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of sodium valproate(VPA) on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flipl, c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibited the growth of SKM-1 cells in dose- and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibited proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles.
