Effect of rhG-CSF on blood coagulation in beagles irradiated by 2.3 Gy neutron.
- Author:
Ming LI
1
;
Qin-Fang HAN
;
Xiao-Lan LIU
;
Shuang XING
;
Guo-Lin XIONG
;
Ling XIE
;
Yan-Fang ZHAO
;
Zu-Yin YU
;
Yi-Bo DING
;
Zhen-Hu ZHAO
;
Yu-Wen CONG
;
Qing-Liang LUO
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Coagulation;
drug effects;
Bone Marrow;
radiation effects;
Dogs;
Granulocyte Colony-Stimulating Factor;
pharmacology;
therapeutic use;
Humans;
Leukocyte Count;
Neutron Diffraction;
Platelet Count;
Radiation Dosage;
Radiation Injuries, Experimental;
physiopathology;
Recombinant Proteins
- From:
Journal of Experimental Hematology
2010;18(6):1520-1524
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effect of recombinant human granulocyte stimulating factor (rhG-CSF) on blood coagulation of beagles irradiated by 2.3 Gy neutron so as to provide new therapy for blood coagulation disorder after neutron irradiation. 10 beagles were exposed to 2.3 Gy neutron, and then randomly assigned into supportive care group and rhG-CSF-treated group. The rhG-CSF-treated cohorts were injected subcutaneously with rhG-CSF (10 µg/kg·d) beginning at the day of exposure for 21 consecutive days. Peripheral blood platelet counts were examined once every two days. In vitro platelet aggregation test, thromboelastography and blood clotting tetrachoric tests were also performed. The results indicated that the blood clotting system of irradiated dogs was in hypercoagulable state in the early days after 2.3 Gy neutron irradiation, and became hypocoagulable at crisis later and were mainly on intrinsic coagulation pathway. Blood fibrinogen increased markedly during the course of disease, while platelet counts and aggregation function were decreased remarkably. rhG-CSF administered daily could correct hypercoagulable state induced by 2.3 Gy neutron irradiation at the early time post exposure, shortened the thromboplastin generation time and clotting formation, down-regulated the abnormal high fibrinogen in blood, and improved platelet aggregation function. It is concluded that rhG-CSF can improve coagulation disorders of irradiated dogs.
