Effect of mesenchymal stem cells transfected with human vegf-165 gene carried by adenovirus on revascularization for hind limb ischemic necrosis in rat model.
- Author:
Yu-Ling LI
1
;
Hong LI
;
Ming-Jun HU
;
Jung-Ming TANG
;
Jia-Ning WANG
Author Information
1. Department of Hematology, Yunyang Medical College, Shiyan 442000, Hubei Province, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Hindlimb;
blood supply;
Humans;
Ischemia;
pathology;
Male;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stromal Cells;
Necrosis;
Neovascularization, Physiologic;
genetics;
Rats;
Rats, Sprague-Dawley;
Transfection;
Vascular Endothelial Growth Factor A;
genetics
- From:
Journal of Experimental Hematology
2010;18(6):1568-1573
- CountryChina
- Language:Chinese
-
Abstract:
The aim of study was to explore the effect of mesenchymal stem cells (MSC) transfected with recombinant adenovirus-mediated human vascular endothelium growth factor 165 (ad-vegf-165) on treating ischemic necrosis limbs. Adult SD rats were selected for study. Limb ischemic necrosis model was established by right femoral artery ligation in SD rats. 7 days after ligation, MSC, ad-h-vegf-165-MSC and ad-LacZ-MSC labelled by DAPI were injected into ischemic necrosis limb in rats respectively. One week after injection, the expression of VEGF in ischemic necrosis limbs was detected by Western blot. And at 1, 2 or 4 weeks after injection, the expressions of FVIII and myosin on MSC were evaluated by immunohistochemistry. The results indicated that the MSC labelled by DAPI could be found in the transplantation site of ischemic necrosis limbs under fluorescent microscope. And the number of MSC in MSC-vegf group was more than that in MSC and MSC-LacZ groups. The VEGF expression in MSC-vegf group was higher than that in MSC and MSC-LacZ groups. More importantly, the number of endothelial cells demonstrated characteristic FVIII positive MSC in MSC-vegf group was more than that in MSC and MSC-LacZ group after injections of 1, 2 and 4 weeks. However, the number of myosin positive MSC among MSC-vegf, MSC and MSC-LacZ groups showed no significant difference. It is concluded that MSC transfected with Ad-vegf promotes angiogenesis to repair ischemic necrosis limbs through the increased expression of VEGF.
