Effect of endomorphin-1 on hematopoietic regulatory molecules from normal human bone marrow stromal cells.
- Author:
Li ZHAO
1
;
Ting LI
;
Lan-Xia ZHOU
Author Information
1. Lanzhou University First Hospital, Lanzhou 730000, Gansu Province, China. zhaoli@lzu.edu.cn
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
drug effects;
metabolism;
Cell Line;
Hematopoietic System;
metabolism;
Humans;
Intercellular Adhesion Molecule-1;
metabolism;
Interleukin-6;
metabolism;
Mesenchymal Stromal Cells;
cytology;
drug effects;
metabolism;
Oligopeptides;
pharmacology;
Tumor Necrosis Factor-alpha;
metabolism;
Vascular Cell Adhesion Molecule-1;
metabolism
- From:
Journal of Experimental Hematology
2010;18(6):1574-1578
- CountryChina
- Language:Chinese
-
Abstract:
The aim of study was to investigate the regulatory effect of endomorphin-1 (EM-1) on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours (p < 0.05), and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no difference in secretion of IL-6 and TNF-α between experimental and control groups (p > 0.05). It is concluded that endomorphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.
