In vitro expansion of T cells stimulated by combination of IL-2, IL-7 and IL-15.
- Author:
Jun DONG
1
;
Su-Xia YANG
;
Yu LI
;
Jiang-Ping GAO
;
Xu ZHANG
Author Information
1. Department of Urology, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Transformed;
Cell Proliferation;
Cell Separation;
Dendritic Cells;
cytology;
metabolism;
Humans;
Interleukin-15;
pharmacology;
Interleukin-2;
pharmacology;
Interleukin-7;
pharmacology;
T-Lymphocytes;
cytology;
drug effects;
metabolism
- From:
Journal of Experimental Hematology
2010;18(6):1590-1594
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to compare cell proliferation and function of the T cells acquired under various culture conditions for establishing a simple, safe and efficient cell expansion protocol in vitro. The peripheral blood mononuclear cells (PBMNC) were isolated and stimulated with autologous dendritic cells (DC) and EBV-transformed B lymphoblastoid cell line (BLCL) weekly. The cell proliferation test, flow cytometry with PI and Annexin V double staining, Cr release test and ELISPOT test were used to detect the cell expansion level, frequency of IFN-γ producing T cells, killing activity of antigen-specific T cells, cell apoptotic status and cell differentiation potential, respectively. The results indicated that use of IL-2 combined with IL-7 and IL-15 resulted in the highest cell expansion comparing to the use of IL-2 alone and the use of CD3/28 Microbeads. Also the cells obtained under cultivating with IL-2, IL-7 and IL-15 together showed high frequency of IFN-γ producing cells, strong killing activity, high viability and high differentiation potential with large portion of CD3(+)CD8(+) population among the T cells. It is concluded that a protocol is established in which the use of IL-2 combined with IL-7 and IL-15 induces the biggest cell expansion, expanded cells show high viability, strong differentiation potential, high frequency of IFN-γ producing cells and strong killing activity.
