Probability of high resolution full match for human leukocyte antigen loci in unrelated donors and recipients with low resolution match.
- Author:
Wei ZHANG
1
;
Fa-Ming ZHU
;
Yan-Min HE
;
Su-Dan TAO
;
Wei WANG
;
Jun-Jun HE
;
Hang-Jun LÜ
;
Li-Xing YAN
Author Information
1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Hangzhou 310006, Zhejiang Province, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Gene Frequency;
Genotype;
HLA Antigens;
genetics;
immunology;
HLA-A Antigens;
genetics;
immunology;
HLA-B Antigens;
genetics;
immunology;
HLA-C Antigens;
genetics;
immunology;
HLA-DQ Antigens;
genetics;
immunology;
HLA-DQ beta-Chains;
HLA-DR Antigens;
genetics;
immunology;
HLA-DRB1 Chains;
Haplotypes;
Hematopoietic Stem Cell Transplantation;
methods;
Histocompatibility Testing;
methods;
Humans;
Probability;
Tissue Donors
- From:
Journal of Experimental Hematology
2010;18(6):1617-1620
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to analyze the possibility of high resolution matching for human leukocyte antigen (HLA) loci in unrelated donor-recipient pair with low resolution match in HLA-A, -B, -DRB1 loci. Samples were genotyped for HLA-A, -B, -C, -DRB1 and -DQB1 by polymerase chain reaction sequence based typing (PCR-SBT). The results showed that the total number of patients and the donors were 166 and 274. 97 (58.43%) patients were matched for 1 donor and 47 (28.31%) patients were matched for 2 donors at low resolution level; among 274 donor-recipient pairs, HLA-A, -B, -C, -DRB1 and -DQB1 loci matching for 6/10, 7/10, 8/10, 9/10 and 10/10 were 32 (11.68%), 54 (19.71%), 62 (22.63%), 49 (17.88%) and 48 (17.52%) respectively; there were mismatch in HLA-A, -B, -C, -DRB1 and -DQB1 loci, and the most mismatch was in HLA-C locus. The number of alleles of HLA-A, -B, -C, -DRB1 and -DQB1 loci were 23, 46, 21, 30 and 17 respectively in the donors. The alleles number HLA-A, -B, -C, -DRB1 and -DQB1 loci were 20, 40, 22, 29 and 16 respectively in the patients; the haplotype number of HLA loci were 311 in the donors and 224 in the patients. The high frequency of haplotype was A*02:07-B*46:01-C*01:02-DRB1*09:01:02-DQB1*03:03 (5.63% and 6.88%). It is concluded that the probability of high resolution mismatch of HLA loci is high in unrelated donor-recipient pairs with low resolution match in HLA-A, -B, -DRB1 loci.
