Detection of single nucleotide polymorphisms of mthfr and dpyd genes in leukemia cell lines K562 and K562/A02.
- Author:
Wen-Jing ZHANG
1
;
Bao-An CHEN
;
Jian CHENG
;
Wen BAO
;
Yue-Jiao ZHONG
;
Feng GAO
;
Guo-Hua XIA
;
Xiao-Ping ZHANG
;
Pei-Pei XU
;
Miao-Xin PENG
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
DNA Mutational Analysis;
DNA Primers;
Dihydrouracil Dehydrogenase (NADP);
genetics;
Drug Resistance, Multiple;
genetics;
Drug Resistance, Neoplasm;
Genotype;
Humans;
K562 Cells;
Methylenetetrahydrofolate Reductase (NADPH2);
genetics;
Polymorphism, Single Nucleotide
- From:
Journal of Experimental Hematology
2011;19(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to detect single nucleotide polymorphisms (SNP) of 2 pharmacokinetics-related genes in K562 and K562/A02 cell lines. Leukemia cell line K562 and its resistant line K562/A02 were cultured, the genomic DNA was isolated by QIAamp DNA Blood Mini kit, primers were designed, the related DNA fragments were amplified by PCR. The SNP genotyping of mthfr gene rs1801131, rs1801133 and rs2274976 and dpyd gene rs1801159, rs1801160 and rs17376848 was performed by means of matrix assisted laser desorption ionization-time of flight mass spectrometry method (MALDI-TOFMS). The results showed that the genotype of mthfr gene locus 1801131 was AC, rs1801133 was CC, rs2274976 was GG, genotype of dpyd gene locus 1801159 was GG, rs1801160 was GG, rs17376848 was AA in both K562 and K562/A02 cell lines. It is concluded that the above-mentioned loci of mthfr and dpyd genes in K562 and K562/A02 cell lines are not expressed differently.
