Relationship between the radiosensitivity of hepatic carcinoma cells and their survivin expression levels.
- Author:
Xiao-jie MA
1
;
Xiao-pin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; radiotherapy; Caspase 3; metabolism; Cell Cycle; radiation effects; Cell Line, Tumor; Gamma Rays; Hep G2 Cells; Humans; Inhibitor of Apoptosis Proteins; Liver Neoplasms; metabolism; radiotherapy; Microtubule-Associated Proteins; metabolism; Up-Regulation
- From: Chinese Journal of Hepatology 2008;16(3):207-209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the radiosensitivity of hepatic carcinoma cells and their survivin expression levels.
METHODSHepatic carcinoma cell lines HepG2 and SMMC-7721 were irradiated with various doses of 60Co gamma-rays. The cell survival rate, expression of survivin, cell cycle profile and activity of caspase-3 were respectively detected by clonogenic assay, immunocytochemistry, flow cytometry and chromatometry.
RESULTSThe surviving fraction at 2Gy (SF2) of HepG2 and SMMC-7721 were 0.43+/-0.01 vs 0.70+/-0.02, and HepG2 had higher radiosensitivity than SMMC-7721. gamma-rays radiation up-regulated the expression of survivin. SMMC-7721 had a significantly higher expression of survivin than HepG2 (t = 2.81-5.20, P < 0.05). The activity of caspase-3 was more powerful in HepG2 than in SMMC-7721 (t = 6.05-6.72, P < 0.01).
CONCLUSIONSurvivin may play a critical role in mediating radiation resistance in SMMC-7721 through its up-regulation and caspase-3 dependent manner.
