Using 1H-MRS to evaluate the effects of warm ischemia reperfusion injury on the regeneration of hepatic cells in orthotopic transplanted livers in rats.
- Author:
Ya-qin ZHANG
1
;
Hong SHAN
;
Qing-cong KONG
;
Jin WANG
;
Xiao-chun MENG
;
Qiang SUN
;
Yang YANG
;
Gui-hua CHEN
;
Hai-fang XING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Graft Survival; Hepatocytes; cytology; Liver Regeneration; Liver Transplantation; Magnetic Resonance Spectroscopy; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury; diagnosis; Warm Ischemia
- From: Chinese Journal of Hepatology 2008;16(4):274-278
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prognostic value of proton magnetic resonance spectroscopy (1H-MRS) in the study of warm ischemia reperfusion injury to the regeneration of hepatic cells of the livers following their orthotopic transplantation in rats.
METHODSA rat orthotopic liver transplantation (OLT) model with warm ischemia, the experimental group, was established and the same was done with a control group but without warm ischemia of the livers. They were studied at 6 time points (6 hours, 1, 3, 7, 14, and 30 days after OLT). All rats took axial T1 weighted and T2 weighted imaging scans and 1H MR spectroscopies.
RESULTSThe positive rate of proliferating cell nuclear antigen (PCNA) and the mean peak choline/water ratio in the experimental group were significantly higher than those in the control group and the peak choline /water ratio had a positive correlation with the positive rate of PCNA. Serum ALT and AST increased significantly after OLT, especially during the 6 hour to 3day period. The levels of ALT and AST were markedly higher in the experimental group compared to the control group.
CONCLUSIONWarm ischemia reperfusion injury of OLT has a significant effect on the regeneration of hepatic cells, and the choline peak of 1H-MRS can be used to evaluate the regeneration of hepatic cells non-invasively.
