The expression of fetuin-A and its pathological significance in fulminant hepatic failure in mice.
- Author:
Pan ZHANG
1
;
Xue-gong FAN
;
Wang HAICHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Proteins; metabolism; Female; Liver; metabolism; pathology; Liver Failure; metabolism; pathology; Mice; Mice, Inbred BALB C; alpha-2-HS-Glycoprotein
- From: Chinese Journal of Hepatology 2008;16(6):440-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the dynamic changes of fetuin-A expression and the influences of the changes on liver damage, hepatocyte apoptosis and inflammation in a mouse fulminant hepatic failure (FHF) model.
METHODSThe changes of fetuin-A expression were investigated by semi-quantitative RT-PCR and Western blot. Immunohistochemical staining was used in TNFa and fetuin-A detection. Hepatocyte apoptosis was detected by TUNEL.
RESULTSFetuin-A mRNA expression decreased after the FHF model was established for 3 hours (compared with the normal group, P less than 0.01), while the protein expression decreased after nine hours (compared with the normal group, P less than 0.01). Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels.
CONCLUSIONIn our mouse FHF model, fetuin-A is a possible protective factor for liver damage.
