Effects of lipid rafts on signal transmembrane transduction mediated by c-Met.
- Author:
Lei WANG
1
;
Yu-feng ZHAO
;
Ya-li LI
;
Yue-fei XU
;
Quan XIA
;
Ke-li MA
Author Information
- Publication Type:Journal Article
- MeSH: Hep G2 Cells; Humans; Membrane Microdomains; metabolism; Mitogen-Activated Protein Kinases; metabolism; Phosphatidylinositol 3-Kinases; metabolism; Phospholipase C gamma; metabolism; Phosphorylation; Proto-Oncogene Proteins c-akt; metabolism; Proto-Oncogene Proteins c-met; metabolism; Signal Transduction
- From: Chinese Journal of Hepatology 2008;16(6):449-452
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of lipid rafts on cell signal transmembrane transduction mediated by c-Met.
METHODSAfter HepG2Cells were treated with MbCD to disrupt the lipid rafts and were treated with artificial recombination hepatocyte growth factor to activate c-Met, the activities of PLCr1/PKC, PI3K/Akt and MAPK signaling pathways in HepG2 cells were analyzed using Western blot.
RESULTS(1) After disruption of lipid rafts with MbCD, phosphorylation of PLCr1 decreased by 35% (P = 0.022); the content of PLCr in the cytoplasm increased by 1.75 fold (P = 0.017); PLCr1 conjugated with membrane decreased by 30% (P = 0.037). (2) The content of PKB in the cytosol decreased by 38% (P = 0.028), and the phosphorylation level of PKB conjugated with membrane decreased by 14% (P = 0.041). At the same time, PDK translocation from cytosol to the plasma membrane and its activation were inhibited by treatment with MbCD. (3) Treatment with MbCD had no significant effect on ErK/MAPK, p38/MAPK and JNK/MAPK signaling pathways.
CONCLUSIONDisruption of lipid rafts with MbCD inhibits the activation of PLCr1/PKC and PI3K/PKB signaling pathways by HGF/cMet, but has no effect on MAPK signaling pathway.
