Relation between mutant p53 and multidrug resistance in gastric cancer.
- Author:
Xin-you XIE
1
;
Ya-jun TAN
;
Yong-liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; genetics; Adenocarcinoma; genetics; Drug Resistance, Multiple; genetics; Humans; Mutation; RNA, Messenger; biosynthesis; genetics; Stomach Neoplasms; genetics; Tumor Cells, Cultured; Tumor Suppressor Protein p53; genetics
- From: Journal of Zhejiang University. Medical sciences 2006;35(1):91-98
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between mutant p53 and multidrug resistance in gastric cancer.
METHODSMutant p53 (mp53) and mp53+sv40Tag were transferred to gastric cancer cell line SGC-7901. The MDR-1 mRNA was examined using RT-PCR, and the difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 was compared with those with mp53+sv40Tag and controls by MTT method.
RESULTSSGC-7901 cells with mutant p53 showed higher MDR-1 mRNA than that of other two groups. SGC-7901 cells with mutant p53 showed higher chemotherapeutic sensitivity to 5-Fu than that with mp53+sv40Tag and control (P<0.05), but no difference between those with mp53+sv40Tag and control (P>0.05). SGC-7901 cells with mutant p53 and those with mp53+sv40Tag showed higher chemotherapeutic sensitivity to ADM than control (P<0.05), but no difference between those with mp53 and with mp53+sv40Tag (P>0.05). There was no difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 compared with those with mp53+sv40Tag and control to CDDP (P>0.05).
CONCLUSIONMutante p53 genes relates to multidrug resistance of gastric cancer.
