Inhibition effect of fludarabine on the growth of myelodysplastic syndrome cell line MUTZ-1 and its mechanism.
- Author:
Wen-jun WU
1
;
Zhen CAI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; biosynthesis; genetics; Myelodysplastic Syndromes; pathology; Neoplasm Proteins; biosynthesis; genetics; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Vidarabine; analogs & derivatives; pharmacology; bcl-2-Associated X Protein; biosynthesis; genetics
- From: Journal of Zhejiang University. Medical sciences 2006;35(2):136-142
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibition effect of fludarabine on the growth of human MDS-RAEB cell line MUTZ-1 and to explore the possible cellular and molecular mechanism.
METHODSThe apoptosis of MUTZ-1 cells induced by fludarabine was studied by transmission electron microscope, MTT assay, DNA ladder test, flow cytometry and RT-PCR method.
RESULTTreatment with fludarabine remarkably inhibited the growth of MUTZ-1 cells, the 24 h IC(50), 48 h IC(50) and 72 h IC(50) of fludarabine for MUTZ-1 cells were 137.65 mg/L, 6.27 mg/L and 0.51 mg/L, respectively. Fludarabine inhibited the growth of MUTZ-1 cells in a dose-dependent and time-dependent manner. After treated by fludarabine (1 mg/L-16 mg/L)for 24 h, MUTZ-1 cells showed the typical features of apoptosis. After fludarabine treatment the mRNA expression of Bcl-2, Bax, survivin, XIAP, cIAP-1 and cIAP-2 was not changed, but the mitochondrial membrane potential (MMP) was decreased.
CONCLUSIONWith a certain range of dose fludarabine (1 mg/L-16 mg/L)could inhibit MUTZ-1 cell growth by inducing cells apoptosis. MMP may play a certain role in apoptosis of MUTZ-1 cells induced by fludarabine.
