Expression of survivin mRNA in HHT-induced cell apoptosis of hematological malignancy cell lines.
- Author:
Han-ying BAO
1
;
Zhen CAI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; HL-60 Cells; Harringtonines; pharmacology; Humans; Inhibitor of Apoptosis Proteins; K562 Cells; Microtubule-Associated Proteins; biosynthesis; genetics; Myelodysplastic Syndromes; metabolism; pathology; Neoplasm Proteins; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2006;35(2):204-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of survivin mRNA in hematological malignancy cells and its correlation with HHT-induced cell apoptosis.
METHODSHematological malignancy cell lines MUTZ-1, K562, Jurkat, RPMI and HL60 were treated with HHT in vitro. Cell apoptosis was observed by flow cytometry (FCM) and the expression of surviving and XIAP mRNA was evaluated with semi-quantitative RT-PCR.
RESULTThe expression of survivin mRNA on cell lines was negatively correlated to HHT-induced cell apoptotic rate (r=-0.980, P=0.003). There were no significant differences in XIAP expression among these 5 cell lines.
CONCLUSIONSurvivin could be used as a new marker for drug-sensitivity and a new target for treatment of hematological malignancies.
