Inhibitory effects of mizolastine on substance P-induced production of leukotriene B4 and interleukin 5 in mouse skin.
- Author:
Yi-na WANG
1
;
Hong FANG
;
Zong-li ZHOU
;
Hang-ping YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzimidazoles; pharmacology; Female; Histamine H1 Antagonists; pharmacology; Interleukin-5; biosynthesis; Leukotriene B4; biosynthesis; Male; Mice; Mice, Inbred BALB C; Skin; metabolism; Substance P; antagonists & inhibitors
- From: Journal of Zhejiang University. Medical sciences 2006;35(2):224-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the inhibitory effect of mizolastine on substance P(SP)-induced production of leukotriene B(4) (LTB(4)) and interleukin 5 (IL-5) in mouse skin.
METHODSMice were fed with different doses of mizolastine or other control drugs, 30 min after administration animals were injected intradermally with SP on the back. The treated skin samples were taken and competitive enzyme-link immunoassay (ELISA) method was applied to detect LTB (4) and IL-5 in the skin samples.
RESULTThe LTB(4) and IL-5 levels in 10 mg/kg mizolastine group were (1.23 +/-0.29)pg/ml and (34.28 +/-11.00)pg/ml, respectively, which were lower than those in saline control group [(5.52+/-1.88)pg/ml and (179.62 +/-46.25)pg/ml respectively] or loratadine group [(3.89+/-1.27)pg/ml and (127.74 +/-43.27)pg/ml respectively]. No significant difference was found between 10 mg/kg mizolastine group and dexamethasone group (P=0.161 and P=0.508).
CONCLUSIONMizolastine might inhibit the production of LTB(4) and IL-5 induced by substance P in mouse skin, suggesting that anti-inflammatory effect and the blockade of histamine H1 receptors might be involved in its anti-pruritic mechanisms.
