VNTR polymorphism of C6orf37 in Chinese population.
- Author:
Jing CUI
1
;
Wei WANG
;
Min WANG
;
Jie LIN
;
Yu MA
;
Wen-jing RUAN
;
Jing XU
;
Mao-de LAI
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; Base Sequence; Chromatography, High Pressure Liquid; methods; Colorectal Neoplasms; genetics; pathology; Exons; genetics; Genetic Predisposition to Disease; genetics; Genotype; Humans; Minisatellite Repeats; genetics; Molecular Sequence Data; Polymorphism, Genetic; genetics; Proteins; genetics
- From: Journal of Zhejiang University. Medical sciences 2006;35(4):354-359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify a novel VNTR in C6orf37 and to detect the C6orf37 VNTR polymorphism distribution in Chinese population.
METHODSRT-PCR and sequencing were conducted to identify VNTR alleles in the variable region of C6orf37.SSLP and DHPLC were applied in detecting the VNTR genotypes in 166 Chinese individuals.
RESULTA novel VNTR sequence was found in the second exon of C6orf37, which was composed of 15 base pairs encoding 5-amino-acid (G-G-D-F-G). The repeat times ranged from 3 to 5. There were three common alleles containing three repeats (a), four repeats (b) and five repeats (c), respectively, which produced three homozygotes (a/a, b/b and c/c) and three heterozygotes (a/b, a/c and b/c). The frequency of a, b, c alleles were 0.145, 0.304, 0.551, respectively in Chinese population. Heterozygosity (H) was 0.583. Polymorphism information content (PIC) was 0.510. The screened result of DHPLC was consistent with that of SSLP.
CONCLUSIONA novel highly polymorphic VNTR in C6orf37 exists in Chinese population. DHPLC is the most efficient technique for screening VNTR polymorphism.