Detection of bcr/abl fusion gene from K562 cell line and mononuclear cells of CML patients by DNA-PCR.
- Author:
Dong XU
1
;
Xun HU
Author Information
- Publication Type:Journal Article
- MeSH: Fusion Proteins, bcr-abl; genetics; Humans; K562 Cells; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Leukocytes, Mononuclear; metabolism; Polymerase Chain Reaction; methods
- From: Journal of Zhejiang University. Medical sciences 2006;35(4):384-389
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect bcr/abl fusion gene at DNA level in K562 cell line and mononuclear cells from patients with chronic myelogenous leukemia (CML).
METHODSBased on previous research, a set of DNA-PCR primers was redesigned. DNA from K562 cells and mononuclear cells of CML patients was extracted respectively. After DNA-PCR for bcr/abl fusion gene the amplified fragments were then sequenced.
RESULTAt DNA level the bcr/abl fusion gene in K562 cells and mononuclear cells of 2 CML patients was amplified. Furthermore, the DNA breakpoint of fusion gene in the above samples through sequencing of amplified fragments was localized.
CONCLUSIONDNA-PCR offers a new detection technology for bcr/abl fusion without the expression of fusion gene.
