Cannabinoid receptor 1 expression and pathological changes in rat hippocampus after deprivation of rapid eye movement sleep.
- Author:
Pei-fang JIANG
1
;
Tao ZHU
;
Zhe-zhi XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hippocampus; metabolism; ultrastructure; Male; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Receptor, Cannabinoid, CB1; biosynthesis; genetics; Sleep Deprivation; metabolism; pathology; Sleep, REM
- From: Journal of Zhejiang University. Medical sciences 2006;35(5):535-540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of cannabinoid receptor 1 (CB1R) mRNA and pathological changes in rat hippocampus after deprivation of rapid eye movement (REM) sleep.
METHODSTotally 42 Sprague-Dawley male rats were randomly divided into cage control (CC), tank control (TC) and the sleep deprivation groups (SD). The SD and TC rats were sacrificed at the end of 1 d, 3 d and 5 d sleep deprivation periods, respectively. The modified multiple platform methods were established for the REM sleep deprivation. CB1R mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). The hippocampus sections of different stages were observed with electron microscope.
RESULTIn SD 1 d group, the expression of CB1R mRNA was significantly increased compared with the CC, TC, SD 3 d and SD 5 d groups (P <0.05) while in SD 3 d group it was reduced. The expression of CB1R mRNA of SD 5 d group was significantly higher than that of the SD 3 d group (P <0.05). Neuron apoptosis was found in SD 3 d and SD 5 d groups.
CONCLUSIONSleep deprivation can cause brain injury with the changes of CB1R mRNA expression.
