IL-10 inhibits inducible expression pattern of beta-defensin-2 in human peripheral blood cells.
- Author:
Qi-xing CHEN
1
;
Jing-lin ZHOU
;
Malte BOOK
Author Information
- Publication Type:Journal Article
- MeSH: Blood Cells; cytology; drug effects; metabolism; Cells, Cultured; Humans; Interleukin-10; pharmacology; Lipopolysaccharides; pharmacology; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; beta-Defensins; genetics
- From: Journal of Zhejiang University. Medical sciences 2006;35(6):596-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of IL-10 on the inducibility of human beta-defensin 2 (hBD-2) in human peripheral blood cells.
METHODSPeripheral blood samples were collected from 22 healthy individuals and co-cultured with 0 ng/ml lipopolysaccharide (LPS), 100 ng/ml LPS, 10 ng/ml IL-10, or 100 ng/ml LPS plus 10 ng/ml IL-10 at 37 degree for 6 h. Total RNA was extracted from peripheral blood cells and the mRNA level of hBD-2 was detected by relative quantitative real-time PCR..
RESULTNo detectable level of hBD-2 mRNA was found in normal peripheral blood cells with stimulation of 0 ng/ml LPS or 10 ng/ml IL-10. The mRNA level of hBD-2 was increased to 166.9 +/- 35.14 after 100 ng/ml LPS challenge, while the mRNA level of hBD-2 was decreased to 30.40 +/- 9.18 after the co-stimulation of 100 ng/ml LPS plus 10 ng/ml IL-10; which was significantly lower than that with LPS alone (P<0.05).
CONCLUSIONThe expression of hBD-2 gene can be induced by LPS.IL-10 inhibits the inducible expression of hBD-2.
